![]() This ISO will then be performed on the newly created experiment (round 2) and such iterations will be done a few more times in a series. For example, if the initial experiment yielded a clear score, the next ISO experiment will increase the precipitant concentration. Based on these scores, the ISO process creates a new experiment wherein the concentration of the precipitant from each condition is altered - either increased or decreased, to get into the nucleation zone in the protein solubility curve. After images are collected on this experiment, a user classifies each of the images by scoring them as either Clear, Crystal, Light Precipitate, or Heavy Precipitate. Using a premade screen, an initial screening experiment is set up. ![]() ISO is a highly automated optimization process that can help researchers yield protein crystals from conditions that initially do not look promising.
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